1. EcoRI under certain conditions possesses cross activity star activity blunt activity polymerase activity
2. Taq polymerase usually adds an adenine cytosine guanine thymine to the end of the polymerized strand of DNA
3. Detection of blotted proteins by antibodies is called a northern southern eastern western
4. The OD 260 value tells about the absorbance emission transmission excitation of DNA at 260 nm wavelength
5. The most common liquid volumes in molecular biology are being measured in ml µl nl l
6. Which form of plasmid DNA runs quickest in an agarose gel open circle linear nicked circle supercoil
7. PCR means Polymerase Chain Reaction Plasmid Coil Removal Point Cross Restriction Primer Circle Reaction
8. A probe is a pointed instrument a labelled piece of nucleic acid a rounded reaction tube a sample of material
9. Reverse transcriptase produces DNA from peptides RNA from DNA DNA from RNA RNA from RNA
10. Competent cells are being used for irradiation evocation transformation purification
11. molecular biologists need ethanol mainly for drinking precipitation of nucleic acids dissolving of nucleic acids precipitation of antibodies
12. A secondary antibody is an antibody that has been used in prior experiments an antibody purchased from a cheap company an antibody that binds to another antibody an antibody that is produced in boostered animals
13. In an agarose gel, DNA is moving from kathode to anode left to right anode to kathode A to B
14. Which of these enzymes produces blunt ends: SaII EcoRV XhoI HindIII
15. A blot is a dried piece of gel a membrane with adherent molecules a square piece of filter paper a failed experiment
